Objective: To found a quantitative HPLC-ELSD method for determining tenuifoliside A

1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucop-yranosyl-(1→2)]-α-D-glucopyranoside and ginsenoside Rb1 from Kaixin San

which are used for anti-tumor. Method: The procedure of HPLC-ELSD was performed on the chromatographi column of Agilent HC C18 (4.6 mm×250 mm

5 μm)at 25 ℃

and the mobile phase was acetonitrile-0.65% ammonium acetate solution in gradient elution.The flow velocity was 1.0 mL · min-1.The temperature of driti tube was 115 ℃ and the gas was air

flow rate was 3.2 L · min-1. Result: Tenuifoliside A showed a good linear relationship at a range from 1.113 μg to 10.980 μg

1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucop-yranosyl-(1→2)]-α-D-glucopyranoside

from 1.581 μg to 15.726 μg

and ginsenoside Rb1

from 0.712 μg to 7.128 μg;Precision

stability and repeatability are comply with the requirement of quantitative analysis. The average recovery was respectively 98.36%

99.58% and 98.97%.The percentage composition was respectively 0.999%

1.670% and 0.579%. Conclusion: The method was simple and reliable

which can be used for the assaying of tenuifoliside A

1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucop-yranosyl-(1→2)]-α-D-glucopyranoside and ginsenoside Rb1 as well as quantity control of Kaixin San.