Objective: To investigate the regulatory effect of Tanyu Tongzhi (TYTZ) recipe on autophagy and antiapoptosis gene of myocardial ischemia-reperfusion injury in rats. Method: Totally 80 SD rats were randomly divided into sham-operated group

model group

3-MA intervention (13.5 mg · kg-1 iv) and TYTZ recipe (43 g · kg-1 ig) groups. The model of ischemia and reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min and reperfusion for 2 hours in rats. Serum contents of CK

LDH were measured by automatic biochemical analyzer. The expression level of Beclin-1

LC3 and Bcl-2 were determined with RT-PCR. Result: Compared with the sham-operated group

the contents of CK

LDH and the expression of Beclin-1

LC3 mRNA were both significantly increased

while the level of Bcl-2 mRNA were decreased in MI and IR model groups (P<0.01). Compared with the model group

the contents of CK and LDH in ischemic period and reperfusion period

the expression of Beclin-1 mRNA in ischemic period and Beclin-1

LC3 mRNA in reperfusion period were lower in TYTZ recipe groups

and myocardial Bcl-2 mRNA activities of the groups in ischemic period and reperfusion period were increased (P<0.05

P<0.01). Compared with the sham-operated group and 3-MA group

the expression of Beclin-1

LC3 mRNA in ischemic period were increased in TYTZ recipe groups (P<0.01). Beclin-1 expression was found to have negative correlation with Bcl-2 expression of time in ischemia and reperfusion periods by Pearson test (P< 0.01). Conclusion: The TYTZ recipe can reduce myocardial ischemia/reperfusion injury by promoting the occurrence of autophagy during ischemia period

inhibiting them in reperfusion and promoting the expression of the anti-apoptotic protein Bcl-2.