龟羚帕安丸对帕金森病大鼠模型中脑黑质细胞Nestin蛋白,Nestin mRNA及DAT蛋白的影响

张良芝; 常学辉; 宁亚红

doi:10.13422/j.cnki.syfjx.2016040115

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龟羚帕安丸对帕金森病大鼠模型中脑黑质细胞Nestin蛋白,Nestin mRNA及DAT蛋白的影响

药理 | 更新时间：2024-01-04

- 龟羚帕安丸对帕金森病大鼠模型中脑黑质细胞Nestin蛋白,Nestin mRNA及DAT蛋白的影响
- Effect of Guiling Pa'an Pill on Expression of Nestin, Nestin mRNA and DAT in Substantia Nigra Cells of Rat Models with Parkinson's Disease
- 中国实验方剂学杂志 2016年22卷第4期页码：115-118
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省中医管理局中医临床学科领军人才培育计划项目(201301006);河南中医学院科技创新人才支持计划项目(2010XCXRC06)
- DOI：10.13422/j.cnki.syfjx.2016040115
  中图分类号： R-332;R285.5
- 纸质出版日期：2016
- 稿件说明：
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张良芝, 常学辉, 宁亚红. 龟羚帕安丸对帕金森病大鼠模型中脑黑质细胞Nestin蛋白,Nestin mRNA及DAT蛋白的影响[J]. 中国实验方剂学杂志, 2016,22(4):115-118.

ZHANG Liang-zhi, CHANG Xue-hui, NING Ya-hong. Effect of Guiling Pa'an Pill on Expression of Nestin, Nestin mRNA and DAT in Substantia Nigra Cells of Rat Models with Parkinson's Disease[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 115-118.
张良芝, 常学辉, 宁亚红. 龟羚帕安丸对帕金森病大鼠模型中脑黑质细胞Nestin蛋白,Nestin mRNA及DAT蛋白的影响[J]. 中国实验方剂学杂志, 2016,22(4):115-118. DOI： 10.13422/j.cnki.syfjx.2016040115.

ZHANG Liang-zhi, CHANG Xue-hui, NING Ya-hong. Effect of Guiling Pa'an Pill on Expression of Nestin, Nestin mRNA and DAT in Substantia Nigra Cells of Rat Models with Parkinson's Disease[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(4): 115-118. DOI： 10.13422/j.cnki.syfjx.2016040115.

摘要

目的: 观察龟羚帕安丸对帕金森病(PD)大鼠黑质细胞巢蛋白(Nestin)

多巴胺转运体(DAT)及Nestin mRNA表达的影响。方法: 大鼠黑质致密部注射6-羟基多巴胺(6-OHDA)

后大鼠ip阿扑吗啡(APO)诱导旋转建立PD大鼠模型。PD大鼠随机分为模型组

多巴丝肼组

龟羚帕安丸高、中、低剂量组

同时设立假手术组

模型组。假手术组每日ig同等容积的生理盐水;多巴丝肼组ig多巴丝肼混悬液(10 mg ·kg-1 ·d-1);龟羚帕安丸高、中、低剂量组ig龟羚帕安丸混悬液(4

1 g ·kg-1 ·d-1)

连续30 d。免疫组化法检测各组大鼠中脑黑质Nestin

DAT蛋白表达

原位杂交法检测各组大鼠中脑黑质Nestin mRNA的表达。结果: 模型组黑质细胞Nestin

DAT蛋白

Nestin mRNA表达均显著低于假手术组(P<0.01);各治疗组Nestin蛋白

Nestin mRNA

DAT蛋白表达均显著高于模型组(P<0.01)。结论: 龟羚帕安丸治疗帕金森病的作用机制可能与上调Nestin

DAT表达

促进神经的修复和再生

增加多巴胺(DA)含量有关。

Abstract

Objective: To investigate the effect of Guiling Pa'an pill on the expression of Nestin

Nestin mRNA and dopamine transporter (DAT) in substantia nigra cells of rats with Parkinson's disease (PD). Method: SPF grade rats were stereotaxically injected with 6-hydrox-ydopamine (6-OHDA) and apomorphinum (APO) to induce PD rat models. Rats were randomly divided into model group

madopar group

Guiling Pa'an pill high dose group

middle dose group and low dose group

and at the same time

the sham operation group was also established. Rats in model group and sham operation group daily received the same volume of normal saline. Rats in madopar group received 10 mg · kg-1 · d-1 suspension by ig. Rats in Guiling Pa'an pill high dose group

middle dose group and low dose group received 4

1 g · kg-1 · d-1 suspension respectively by ig

all with a course of 30 days. The Nestin and DAT protein levels of the substantia nigra tissue were detected by immunohistochemistry for various groups;Nestin mRNA level in substantia nigra tissues was detected by hybridization in situ method for various groups. Result: Nestin and DAT protein expression and Nestin mRNA expression in substantia nigra tissues of the model group were significantly lower than those in sham operation group (P<0.01). Compared with model group

the Nestin protein

Nestin mRNA and DAT protein expressions in substantia nigra tissues of various treatment groups were significantly increased (P<0.01). Conclusion: Action mechanism of Guiling Pa'an pill for the treatment of Parkinson's disease might be associated with increasing Nestin

DAT expressions

which would promote the repair and regeneration of nerves and increase the content of dopamine.

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