超滤法结合替代对照品HPLC测定大蒜辣素与大鼠及人血浆的蛋白结合率

马雪红; 王健; 耿晶; 李新霞; 陈坚

doi:10.13422/j.cnki.syfjx.2016050105

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超滤法结合替代对照品HPLC测定大蒜辣素与大鼠及人血浆的蛋白结合率

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- 超滤法结合替代对照品HPLC测定大蒜辣素与大鼠及人血浆的蛋白结合率
- Determination of Human and Rat Plasma Protein Binding of Allicin by Ultrafiltration and HPLC with Alternative Reference
- 中国实验方剂学杂志 2016年22卷第5期页码：105-109
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81260486)
- DOI：10.13422/j.cnki.syfjx.2016050105
  中图分类号： O657.72;R965
- 纸质出版日期：2016
- 稿件说明：
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马雪红, 王健, 耿晶, 等. 超滤法结合替代对照品HPLC测定大蒜辣素与大鼠及人血浆的蛋白结合率[J]. 中国实验方剂学杂志, 2016,22(5):105-109.

MA Xue-hong, WANG Jian, GENG Jing, et al. Determination of Human and Rat Plasma Protein Binding of Allicin by Ultrafiltration and HPLC with Alternative Reference[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(5): 105-109.
马雪红, 王健, 耿晶, 等. 超滤法结合替代对照品HPLC测定大蒜辣素与大鼠及人血浆的蛋白结合率[J]. 中国实验方剂学杂志, 2016,22(5):105-109. DOI： 10.13422/j.cnki.syfjx.2016050105.

MA Xue-hong, WANG Jian, GENG Jing, et al. Determination of Human and Rat Plasma Protein Binding of Allicin by Ultrafiltration and HPLC with Alternative Reference[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(5): 105-109. DOI： 10.13422/j.cnki.syfjx.2016050105.

摘要

目的: 测定大蒜辣素与大鼠及人血浆的蛋白结合率

为大蒜辣素的成药性研究提供参考. 方法: 以羟苯乙酯为替代对照品

采用C18色谱柱

流动相甲醇-1%甲酸梯度洗脱

流速1.0 mL ·min-1

检测波长254 nm

结合超滤法对大蒜辣素与大鼠及健康人血浆的蛋白结合率进行测定. 结论: 测得75

150

300 mg ·L-1大蒜辣素溶液与大鼠和人血浆蛋白结合率分别为23.2%

19.8%

28.1%及15.5%

25.8%

40.0%. 结论: 大蒜辣素与大鼠及人的血浆蛋白结合率均较低

替代对照品HPLC可用于测定大蒜辣素的血浆蛋白结合率.

Abstract

Objective: To Detemine human and rat plasma protein binding rate of allicin

and provide a reference for research of allicin into medicinal properties. Method: With ethylparaben as an alternative reference

C18 column was used

mobile phase was methanol-1% formic acid for gradient elution in a flow rate of 1.0 mL · min-1 and detection wavelength was 254 nm. Result: With different concentration of allicin including 75

150

300 mg · L-1

protein binding rates of allicin in rat and human plasma were 23.2%

19.8%

28.1% and 15.5%

25.8%

40.0%

respectively. Conclusion: Allicin has low level in binding to plasma protein of human and rat.HPLC with ethylparaben as alternative reference can be used to determine plasma protein binding rate of allicin.

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