Objective: To discuss the interventional effect of Shuanghe Tang on alcohol-induced osteonecrosis of the femoral head (AOFH). Method: Totally 60 Chinese white rabbits were randomly divided into the normal group

the model group

the madopar group

and low

medium and high-dose Shuanghe Tang groups. Except for the normal group

all of the remaining groups were lavaged with Chinese liquor (alcohol content 56%) 10 mL · kg-1 · d-1. Meanwhile

each drug-treated group was lavaged with drugs. The normal group and the model group were given normal saline. After 8 weeks of reventive administration

the rabbits were sacrificed to collect blood for determination of the index of hemorheology

serum total cholesterol (TC)

triglyceride (TG)

high density lipoprotein (HDL)

low density lipoprotein (LDL)

bone glaprotein (BGP)

vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2). The rabbits were killed after blood collection

and histopathological changes were detected. The expression levels of PPARγ mRNA and OC mRNA in femoral heads were analyzed by RT-PCR. Result: Compared with the normal group

the level of TC

TG

LDL and the index of hemorheology in the model group were significantly increased(P<0.01)

and the levels of HDL

BGP

VEGF and BMP-2 were significantly decreased (P<0.01). Compared with the model group

the level of TC

TG

LDL and the index of hemorheology were significantly decreased in the medium and high-dose Shuanghe Tang groups

and the level of HDL

BGP

VEGF

BMP-2 and Osteoblast count were significantly increased (P<0.01). Compared with the normal group

the empty bone lacuna rate and average diameter of the max adipocyte in the model group were significantly increased(P<0.01)

and the osteoblasts count was significantly decreased (P<0.01). Compared with the model group

the empty bone lacuna rate and average diameter of the max adipocyte were significantly decreased in the medium and high-dose Shuanghe Tang group (P<0.01)

and the osteoblasts count was significantly increased (P<0.01). Compared with the normal group

the expression of PPARγ mRNA in the model group was significantly increased (P<0.01)

and the expression of OC mRNA was significantly decreased (P<0.01). Compared with the model group

the expression of PPARγ mRNA was significantly decreased in the medium dose Shuanghe Tang group (P<0.01)

and the expression of OC mRNA was significantly increased(P<0.0). Conclusion: Shuanghe Tang can effectively relieve alcohol-induced hyperlipidemia

improve hemorheology and reduce blood viscosity

inhibit adipogenic differentiationof marrow stromal cells induced by alcohol

and maintain osteogenic differentiation in femoral head in rabbits

so as to prevent and treat AOFH.