Objective: To study the effect and mechanism of oridonin on inhibiting angiogenesis. Method: In vitro and in vivo models were used in this paper to study the anti-angiogenic effects of oridonin. Through in vitro models

methyl thiazolyl tetrazolium (MTT) assay was ysedti detect the cells vitality and enzyme-linked immunosorbent assay (ELISA) was used to determine vascular endothelial growth factors level (VEGF) in cardiac microvascular endothelial cells (CMEC). Through in vivo models

transgenic zebrafish models (Fli1-GFP) were used to observe the effect of oridonin on angiogenesis of embryonic period and regeneration after vascular injury in adult zebrafish. Expressions of main related genes VEGFA

VEGFR2 and VEGR3 in VEGF pathways were detected with relative fluorescence quantitative PCR method. Result: Oridonin inhibited angiogenesis and endothelial cells in vitro

with IC50 of 8.04 mg · L-1

and significantly reduced VEGF expression in the cell serum. What's more

oridonin inhibited angiogenesis in zebrafish embryos vascular assay and inhibited regeneration after vascular injury in adult zebrafish. Our study elucidated the mechanism of the anti-angiogenic activity of oridonin at least in part

which may be related to down-regulating the expression of VEGFA

VEGFR2 and VEGR3 genes. Conclusion: Oridonin can effectively inhibit angiogenesis

which will provide greater contribution for anti-tumor angiogenesis therapy.