Objective: To investigate the effects of capsaicin on the invasion ability of human large cell carcinoma NCI-H460 and the expressions of E-cadherin and Slug

in order to discuss the possible mechanisms of anti-non-small cell lung cancer. Method: NCI-H460 cells were cultured in vitro and treated with capsaicin with various concentrations

with no capsacin in the blank group. The 50% inhibitory concentration(IC50) was measured by MTT method. The NCI-H460 cell invasion ability was evaluated by actin-tracker green fluorescent probe and transwell chamber invasion assay. Western blot and RT-PCR were used to detect the protein and mRNA expression of E-cadherin and Slug. Result: MTT assay results showed that the growth of NCI-H460 cells treated with capsaicin was significantly inhibited compared with the blank group

with statistically significant difference (P<0.05);Actin-tracker Green fluorescent probe showed that capsaicin can notably prohibit NCI-H460 filopodia formation. Transwell invasion in vitro results showed that capsaicin can significantly inhibit invasion of penetrating cells

with statistically significant difference compared with the control group (P<0.05). Western blot and RT-PCR analysis showed that E-cadherin expression level was significantly elevated and Slug expression level was significantly decreased by capsaicin

with statistically significant difference compared with the control group (P<0.05). Conclusion: Capsaicin can inhibit the NCI-H460 cell viability and invasion

its mechanism for anti-non-small cell lung cancer may be correlated with decrease in Slug expression and increase in E-cadherin expression.