HPLC同时测定炎热清软胶囊中栀子苷、黄芩苷和汉黄芩苷的含量

龙厚宁; 印酬; 孟小夏; 张敏; 王鹏娇; 高秀丽

doi:10.13422/j.cnki.syfjx.2016060034

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HPLC同时测定炎热清软胶囊中栀子苷、黄芩苷和汉黄芩苷的含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定炎热清软胶囊中栀子苷、黄芩苷和汉黄芩苷的含量
- Simultaneous Detemination of Geniposide, Baicalin and Wogonoside in Yanreqing Soft Capsules by HPLC
- 中国实验方剂学杂志 2016年22卷第6期页码：34-37
- 作者机构：
- 作者简介：
- 基金信息：
  
  贵州省普通高校微生物与生化药学工程研究中心项目(黔教合KY字[2015]338)
- DOI：10.13422/j.cnki.syfjx.2016060034
  中图分类号： O657.72;R286.0
- 纸质出版日期：2016
- 稿件说明：
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龙厚宁, 印酬, 孟小夏, 等. HPLC同时测定炎热清软胶囊中栀子苷、黄芩苷和汉黄芩苷的含量[J]. 中国实验方剂学杂志, 2016,22(6):34-37.

LONG Hou-ning, YIN Chou, MENG Xiao-xia, et al. Simultaneous Detemination of Geniposide, Baicalin and Wogonoside in Yanreqing Soft Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 34-37.
龙厚宁, 印酬, 孟小夏, 等. HPLC同时测定炎热清软胶囊中栀子苷、黄芩苷和汉黄芩苷的含量[J]. 中国实验方剂学杂志, 2016,22(6):34-37. DOI： 10.13422/j.cnki.syfjx.2016060034.

LONG Hou-ning, YIN Chou, MENG Xiao-xia, et al. Simultaneous Detemination of Geniposide, Baicalin and Wogonoside in Yanreqing Soft Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(6): 34-37. DOI： 10.13422/j.cnki.syfjx.2016060034.

摘要

目的: 建立HPLC同时测定炎热清软胶囊中栀子苷、黄芩苷和汉黄芩苷的含量。方法: 样品采用石油醚回流脱脂

70%甲醇超声提取30 min。采用Diamonsil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.4%磷酸溶液梯度洗脱

流速1.0 mL·min-1

柱温30 ℃

检测波长240 nm。结果: 炎热清软胶囊中3种有效成分的分离度良好

栀子苷、黄芩苷和汉黄芩苷的平均回收率分别为100.63%

100.77%和97.67%

RSD分别为1.9%

1.5%和1.1%

(n=6)。结论: 该方法简便准确、灵敏度高、专属性强

重复性好

可用于炎热清软胶囊的质量控制。

Abstract

Objective: To establish a HPLC method to determine geniposide

baicalin and wogonoside in Yanreqing soft capsules. Method: The samples were degreased with petroleum ether and extracted with 70% methanol for 30 min in a ultrasonic mode. Diamonsil C18column (4.6 mm×250 mm

5 μm) was eluted with the mobile phase of acetonitrile-0.4% phosphoric acid in a gradient mode. The flow rate was 1.0 mL·min-1

the column temperature was 30 ℃

and the detection wavelength was set at 240 nm. Result: The average recoveries were 100.63% for geniposide with RSD of 1.9%;100.77% for baicalin with RSD of 1.5%;and 97.67% for wogonoside with RSD of 1.14%. Conclusion: The method is simple

accurate

highly sensitive

specific and repeatable. It can be used for the quality control of Yanreqing soft capsules.

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