Objective: To compare the similarities and differences of Atractylodes chinensis rhizome in chemical constituents between different origins:Chengde of Hebei

Inner Mongolia

Liaoning

Jilin and Shanxi. Method: Gas chromatography-tandem quadrupole mass spectrometer (GC-MS) was used to analyze the A. chinensis from different origins;qualitative identification was done for various chemical constituents

followed by content comparison and cluster analysis between different samples. Result: A total of 35 chemical compounds were detected in all of the A. chinensis rhizome samples from different origins

and 33 of them were qualitatively identified

including 13 kinds of olefins

9 kinds of alcohols

6 kinds of ketones

5 kinds of esters and 2 kinds of aromatic hydrocarbons. Cluster analysis results showed that A. chinensis from Chengde and A. chinensis rhizome from Inner Mongolia were classified into one category;A. chinensis rhizome from Liaoning

Jilin and Shanxi were classified into the other category. Conclusion: A. chinensis originated from five different regions above can be divided into two categories;A. chinensis rhizome from Chengde is similar with the one from Inner Mongolia in the respect of chemical compositions.