Objective: To discuss the mechanism of Yiqi Yangyin formula on mitochondrial pathway of granulosa cells apoptosis induced by cDDP. Method: Granulosa cells apoptosis model was induced by cDDP. Then drug-containing serum of different concentrations (10.5

21

31.5 g·kg-1) were used to intervene in apoptosis. The blank group was also set up. The proliferation and apoptosis were analyzed by MTT assays and flowcytometry. GC apoptosis rate was detected with TUNEL staining. Calcium concentration and mitochondrial membrane potential were detected by confocal laser scanning microscope. The mRNA expressions of Bcl-2

Bad

Caspases-3 and Caspases-9 were detected by RT-PCR;protein levels of PI3K

Akt and p-Akt were detected by western blot

respectively. Result: Compared with the blank group

the model group showed significant increase in GC apoptosis rate and Ca2+ fluorescence intensity and decrease in mitochondrial membrane potential(MMP). Besides

the mRNA expressions of Bad

Caspases-3

Caspases-9 were significantly higher in model group than those in control group (P<0.05). Meanwhile

Bcl-2 mRNA expression

PI3K and p-Akt were significantly lower in model group (P<0.05). In middle and high dose groups

calcium concentration

the mRNA expression of Bcl-2

Caspase-3 and Caspase-9 were down-regulated significantly (P<0.05)

while the mitochondrial membrane potential

Bcl-2 mRNA expression

and the protein levels of PI3K and p-Akt were up-regulated significantly (P<0.05). Conclusion: Yiqi Yangyin formula can inhibit GC apoptosis induced by cDDP by activating PI3K/Akt signal pathway

up-regulating anti-apoptosis factors (Bcl-2)

down-regulating expressions of pro-apoptosis factors (Bad and caspases family)

stabilizing the mitochondrial membrane potential and reducing calcium influx.