Objective: To observe the effect of Fushen Jiangzhuo decoction (FJD) on biochemical indicators

kidney tissue morphology

expression of fibrosis factor transforming growth factor-β1 (TGF-β1)

hepatocyte growth factor (HGF) and collagen type Ⅰ (ColⅠ) protein in rats with renal interstitial fibrosis. Method: On the base of mesangial proliferative glomerulonephritis (MsPGN)

the modeling time was extended to 12 weeks

making natural development of renal interstitial fibrosis in rats. Totally 24 rats were randomly divided into normal group

model group

FJD Chinese medicine group and benazepril group. Blood urea nitrogen (BUN)

serum creatinine (SCr)

and 24 hours urinary protein in serum were detected in the rats. Light microscope was used to observe renal biopsy tissues. The protein expressions of TGF-β1

HGF and ColⅠ in renal tissues were detected respectively by immunohistochemistry assay. Result: Through the observation of BUN

SCr

24 hours urinary proteins and renal tissue morphology

obvious renal interstitial injury was obvious in model group when compared with the normal group (P<0.01)

and the injury degree was alleviated in benazepril group and the Chinese medicine group (P<0.01). At the end of 12 weeks

expressions of TGF-β1 and ColⅠin modeling groups were higher than those in normal group (P<0.01)

but were significantly decreased in FJD group and benazepril group (P<0.01). Level of HGF protein expression in model group was lower than that in normal group (P<0.01)

but was increased in FJD group and benazepril group (P<0.01). Conclusion: FJD can protect renal interstitial fibrosis by inhibiting TGF-β1and ColⅠprotein expressions and increasing HGF protein expression.