Objective: To investigate the chemical constituents of n-butanol fraction of notoginseng medicinal fungal substance. Method: The compounds were separated and purified through the system solvent extraction

D101 macroporous adsorbent resin

silica gel column chromatography

open ODS column chromatography

and semi-preparative liquid chromatography. The structures of the compound were identified by chemical properties and spectroscopic data. Result: Six compounds were separated from n-butanol fraction of notoginseng medicinal fungal substance and identified as ginsenoside Rk3(1)

pseudoginsenoside RT2(2)

ginsenoside Rg3(3)

24(R)-pseudoginsenoside GQ (4)

ginsenoside Rd (5)

and gypenoside LXIX (6). Conclusion: Compound 2 and compound 4 were isolated from notoginseng for the first time

and all compounds were isolated from notoginseng medicinal fungal substance for the first time.