Objective: To investigate the chemical constituents of n-butanol fraction of notoginseng medicinal fungal substance which was obtained from notoginseng by biotransformation technology. Method: The compounds were isolated and purified through system solvent extraction

D101 macroporous adsorptive resin

silica gel column chromatography

open ODS column chromatography

and semi-preparative liquid chromatography. The structures of these isolated compounds were identified by means of chemical properties

spectroscopic analysis. Result: Six compounds were separated from n-butanol fraction of notoginseng medicinal fungal substance and identified as 24(R)-pseudoginsenoside RT5(1)

ginsenoside SG1(2)

ginsenoside Rh12(3)

ginsenoside Rb1(4)

vina-ginsenoside R8(5)

and vina-ginsenoside R9(6). Conclusion: Compounds 1

2

5 and 6 were isolated from this plant for the first time

and all compounds were isolated from notoginseng medicinal fungal substance for the first time.