Objective: To establish an HPLC method for simultaneous determination of ginsenoside Rg1

ginsenoside Re

ginsenoside Rf

ginsenoside Rh1

ginsenoside Rb1

ginsenoside Ro

ginsenoside Rb2

and ginsenoside Rd in ginseng medicinal fungal substance. Method: High performance liquid chromatography was carried out on Inertsil ODS-SP chromatographic column (4.6 mm×250 mm

5 μm) with acetonitrile-0.4% phosphate as the mobile phase for gradient elution. The flow rate was 1 mL · min-1

and detection wavelength was 203 nm. The column temperature was at 30 ℃. Result: Eight kinds of monomer saponins could be separated well in 100 min

with good linear relationship (R2>0.999 7).The average recovery rate was 100.15% (RSD 1.6%)

101.54% (RSD 2.3%)

101.87% (RSD 1.9%)

100.88% (RSD 2.1%)

102.10% (RSD 1.5%)

102.84% (RSD 0.8%)

101.38% (RSD 2.7%)

and 101.23% (RSD 1.6%). Conclusion: The HPLC method is accurate

reliable and reproducible to analyze 8 saponins in ginseng medicinal fungal substance

which can provide the scientific basis for formulating the quality standard of the research on composition transformation of the ginseng medicinal fungal substance.