Objective: To study the effect of compatibility of aconitine and ginsenosides Rb1 on ATP enzyme and relatedion of myocardial cells. Method: Tryptic digestion and differential adherence method were used to culture myocardial cells in new born rats

and then these rats were divided into normal group

aconitine group

ginsenosides Rb1group

and aconitine and ginsenosides Rb1 compatibility (1 : 1

1 : 2

2 : 1) groups. After treatment for one hour

the activity of myocardial cells

ion concentration and ATP enzyme activity in myocardial cells were detected in various groups. Result: The 0.2% aconitine could reduce myocardial cells activity

reduce myocardial intracellular Mg2+ and K+ concentration

increase concentration of Ca2+ and Na+

and reduce Ca2+-ATPase

Na+-K+ ATPase and Ca2+-Mg2+-ATPase activities;compatibility of aconitine and ginsenosides Rb1 could increase myocardial intracellular Mg2+ and K+ concentration

reduce myocardial intracellular Ca2+ and Na+ concentration

and improve Ca2+-ATPase

Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities. Conclusion: Ginsenosides Rb1 can reduce the aconitine toxicity on cardiomyocytes

and mechanism may be associated with regulating ATPase activity of myocardial cell membrane and related ion concentration in cells.