Objective: To observe the effect of different concentrations of Shuanghuanglian injection (SHLI) and its intermediates on proliferation of RBL-2H3 cells

and provide evidence for establishing anaphylactoid cell models and monitoring drug quality. Method: RBL-2H3 cells were treated with different concentrations of SHLI (2 times gradient dilution from volume ratio 1: 16 to 1: 4 096) and its intermediates (2 times gradient dilution from volume ratio 1: 8 to 1: 2 048). The cellular morphology was observed under inverted microscope. MTT colorimetric assay was used to determine the growth of RBL-2H3 cells in different concentration of SHLI and its intermediates

and the half inhibitory concentration (IC50) was calculated. Dependent on the value of IC50

RBL-2H3 cells were then treated with different concentrations of SHLI to observe the effect of SHLI on cells apoptosis. Result: As the concentration increases of SHLI and its intermediates

their inhibitory effect on proliferation of RBL-2H3 cells was gradually increased; the cells survival rate was decreased significantly; cells injury was gradually aggravated; and the cellular morphology was also changed. High dose of SHLI could induce apoptosis of RBL-2H3 cells. Conclusion: SHLI and its intermediates could reduce RBL-2H3 cells proliferation

we can provide certain experiment basis for establishing anaphylactoid cell models and monitoring drug quality and safety.