Objective: To observe the effect of Huoxue Qushi formula on the non-alcoholic steatohepatitis (NASH) model rat and explore it possible mechanism. Method: All Wistar rats of NASH model were induced by feeding high fat diet for 8 weeks

and they were randomly divided into the rogridone group(3 mg · kg-1 · d-1)

the low

middle

high dose Huoxue Qushi formula groups (8

16

32 g · kg-1 · d-1)

the model and the normal group. After 4-week intervention

the levels of alanine aminotransferase (ALT)

aspartate amino transferase (AST)

triglyceride (TG)

total cholesterol (TC)

high density lipoprotein cholesterol (HDL-C) were determined by a colorimetric method. The levels of fasting blood-glucose (FBG) and fasting insulin (FINS) were determined by radioimmunoassay. The levels of tumor necrosis factor-α (TNF-α)

interleukin (IL )-10 and IL-6 were determined by ELISA. The activity of superoxide dismutase (SOD) and the content of malonaldehyde (MDA) were determined. The liver pathology was observed by HE staining and peroxisome proliferator-activated receptor α(PPARα) mRNA expression of liver homogenate was tested by RT-PCR. The content and distribution of CYP2E1 were tested by the ABC method. Result: Compared to the normal group

the levels of ALT

AST

TG

TC

FBG

FINS

TNF-α and IL-6 increased

the levels of HDL-C and IL-10 decreased

the SOD activity reduced

the contents of MDA and CYP2E1 increased and the PPARα mRNA expression reduced in the model group (P<0.05). Compared with model group

all the above indexes and the liver pathology were improved in the Huoxue Qushi formula groups (P<0.05). Pathological results showed that the liver tissue injury obviously improved. Conclusion: The Huoxue Qushi formula has certain effect in effectively treating NASH through many ways involved in improving insulin resistance

reducing lipid peroxidation and inhibiting the inflammatory reaction.