Objective: To identify Physalis plants in Solanaceae by internal transcribed spacer 2(ITS2) sequence. Method: ITS2 sequence of Physalis and Physaliastrum medicinal plant samples was amplified by RT-PCR and sequenced.In order to expand scope of research topic

ITS2 sequences of related species were downloaded from GenBank.All sequences were spliced by CodonCode Aligner V3.0.1

ITS2 sequences were compared and analyzed by DNAMAN software.Related data analysis and processing was performed by MEGA 5.10 software and neighbor-joining tree was constructed.ITS2 secondary structure information was predicted by ITS2 database and its differences of samples was analyzed. Result: All samples were clustered into two branches

Physaliastrum was clustered into a single branch and the same as Physalis.Except a few samples

all species of both genus were clustered into a branch where all intra-species samples were clustered to one branch respectively and obviously distinguished

which showed monophyletic.Bootstrap support rates between each two were very high. Conclusion: ITS2 sequences have great potential in terms of systematic research and species identification between related species.