Objective: To investigate influence of different drying method on contents of antitumor ingredients in fresh leaves of Paliurus ramosissimus and optimize its extraction process. Method: HPLC-ELSD was adopted to determine contents of betulinic acid and paliurusene with mobile phase of 1% formic acid-acetonitrile with gradient elution and detection wavelength of 320 nm.Take comprehensive score of contents of betulinic acid and paliurusene as index

othogonal test was adopted to optimize extraction process with ethanol concentration

ethanol comsumption and extracting time as factors. Result: It was better for conservation of betulinic acid and paliurusene by freeze drying and drying in shade.Optimal extraction conditions was as follows:extracted twice with 4 times the amount of 95% ethanol

48 h for per time;yield of betulinic acid and paliurusene were up to 4.61

0.0774 mg·g-1. Conclusion: Freeze drying is the best drying method for P. ramosissimus

but considering cost and mass production

drying in shade is better.This optimized extraction process is stable and reasonable with high yield of active constituents.It can provide a reference for development and utilization of P. ramosissimus.