Objective: To identify and explore the antimicrobial activity of the fungi (Cs-ZZ23) isolated from the Cordyceps sinensis collected from Guoluo

Qinghai province. Method: The morphology and multilocus phylogenetic analysis were used for identification of the fungi. Meanwhile

6 strains were used as indicators to test the antimicrobial activity of this fungus using agar disk diffusion method and improved agar block method. Result: The morphological characteristics of this strain were similar to those of Mortierella alpina in M. sp.

and the polygenes sequence analysis showed that the sequence homology was 99% between strain Cs-ZZ23 and the multiple strains of M. alpina.Therefore

this strain was determined as M. alpina and it exhibited antibacterial activity against E. coli

Bacillus subtilis and Pythium aphanidermatum. Conclusion: The strain Cs-ZZ23 is a kind of M. alpina and its secondary metabolites deserve to be further developed.