Objective: To improve preparation process of Jinying Huanggui decoction for increasing the content of total polysaccharides in this preparation. Method: Step of petroleum ether extraction in preparation process of Jinying Huanggui decoction had been deleted

changed 70%

75% and 80% alcohol precipitation step to 80% alcohol precipitation by one step.Monosaccharide composition in total polysaccharides from Jinying Huanggui decoction was analyzed by GC-MS.Comparison of the content of total polysaccharides before and after process improvement was determined by phenol-sulfuric acid colorimetry. Result: Monosaccharide composition in total polysaccharides was composed of rhamnose

arabinose

xylose

mannose

glucose and galactose with molar ratio of 0.08:0.08:0.02:0.03:1.00:0.04;after process improvement

this molar ratio was 0.05:0.07:0.02:0.05:1.00:0.08.Before and after process improvement

average concentrations of total polysaccharides in Jinying Huanggui decoction were 11.52

22.69 g·L-1

respectively. Conclusion: This method is simple

accurate and reproducible

it can be used for determination of total polysaccharides of Jinying Huanggui decoction.Process improvement of this preparation is in favor of increasing of total polysaccharides content.