Objective: To establish a method for rapid determination of the content of buddleoside in Chrysanthemi Indici Flos by Near Infrared Spectroscopy (NIRS) and partial least square (PLS). Method: The near infrared spectra of Chrysanthemi Indici Flos were collected. With the content of buddleoside determined by HPLC method as a reference value

the NIR spectra were then associated with the reference values to establish the quantitative prediction model of buddleoside in Chrysanthemi Indici Flos. Finally

the model was verified by unknown samples. Result: The correlation coefficients (R2) of the quantitative analysis model was 0.99909;the root mean square error of calibration (RMSEC) was 0.0136;the root mean square error of cross validation (RMSECV) was 0.03970;the correlation coefficients of predication (r) and the root mean square error of predication (RMSEP) was 0.9976 and 0.0185 respectively. Conclusion: This method of near infrared spectroscopy is simple

fast and accurate

and could be used for the rapid determination of the content of the buddleoside in Chrysanthemi Indici Flos of Henan province.