菝葜抗炎有效部位群在大鼠肠外翻试验中的吸收特性考察

黄慧辉; 张小燕; 沈娟; 罗丹; 叶晓川; 刘焱文

doi:10.13422/j.cnki.syfjx.2016080096

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菝葜抗炎有效部位群在大鼠肠外翻试验中的吸收特性考察

药物代谢 | 更新时间：2024-01-04

- 菝葜抗炎有效部位群在大鼠肠外翻试验中的吸收特性考察
- Investigation of Absorption Characteristics of Anti-inflammatory Effective Parts from Smilacis Chinae Rhizoma in Rat Intestinal Valgus Test
- 中国实验方剂学杂志 2016年22卷第8期页码：96-99
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(31370378)
- DOI：10.13422/j.cnki.syfjx.2016080096
  中图分类号： R285.5
- 纸质出版日期：2016
- 稿件说明：
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黄慧辉, 张小燕, 沈娟, 等. 菝葜抗炎有效部位群在大鼠肠外翻试验中的吸收特性考察[J]. 中国实验方剂学杂志, 2016,22(8):96-99.

HUANG Hui-hui, ZHANG Xiao-yan, SHEN Juan, et al. Investigation of Absorption Characteristics of Anti-inflammatory Effective Parts from Smilacis Chinae Rhizoma in Rat Intestinal Valgus Test[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 96-99.
黄慧辉, 张小燕, 沈娟, 等. 菝葜抗炎有效部位群在大鼠肠外翻试验中的吸收特性考察[J]. 中国实验方剂学杂志, 2016,22(8):96-99. DOI： 10.13422/j.cnki.syfjx.2016080096.

HUANG Hui-hui, ZHANG Xiao-yan, SHEN Juan, et al. Investigation of Absorption Characteristics of Anti-inflammatory Effective Parts from Smilacis Chinae Rhizoma in Rat Intestinal Valgus Test[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(8): 96-99. DOI： 10.13422/j.cnki.syfjx.2016080096.

摘要

目的: 考察菝葜抗炎有效部位群中6种成分的体外肠吸收特征

为该部位的制剂开发及药代动力学研究提供参考。方法: 采用大鼠肠外翻模型

通过HPLC测定不同取样时间肠内液中白藜芦醇苷、落新妇苷、氧化白藜芦醇、槲皮苷、黄杞苷、白藜芦醇的含量

流动相乙腈(A)-0.1%磷酸水溶液(B)梯度洗脱(0~18 min

12%~18%A;18~33 min

18%A;33~45 min

18%~21%A;45~50 min

21%A)

流速1 mL·min-1

检测波长290 nm

计算吸收动力学参数

考察6个成分在不同肠段(十二指肠、空肠和回肠)的吸收情况。结果: 白藜芦醇苷在各肠段吸收无明显差异

而其他5种成分在各肠段的吸收差异较大;6种成分在同一肠段的吸收有较大差异

在十二指肠、空肠和回肠中的吸收率分别为0.85%~2.79%

0.70%~2.73%和0.90%~1.77%。槲皮苷在十二指肠和空肠的吸收率最高

白藜芦醇苷在回肠的吸收率最高;氧化白藜芦醇吸收速度明显比其他5种成分慢

在十二指肠中于90 min后才表现出较明显的吸收。结论: 白藜芦醇苷、落新妇苷、槲皮苷、黄杞苷、白藜芦醇在大鼠离体小肠的吸收为一级动力学过程

选择外翻肠囊法可评价菝葜提取物中多成分的肠吸收特性。

Abstract

Objective: To investigate absorption characteristics of six components from anti-inflammatory effective parts of Smilacis Chinae Rhizoma. Method: Taking rat everted gut sac model

intestinal sac contents were collected at different time and absorption concentration of polydatin

astilbin

oxyreverstroal

quercitrin

engeletin and resveratrol was determined by HPLC

absorption kinetics parameters were calculated for describing their absorption characters in different intestinal segments (duodenum

jejunum and ileum). Result: Absorption of polydatin in duodenum

jejunum and ileum was no significant difference

while the other five components showed large differences.Absorption of six ingredients showed significant differences in the same intestinal site

their absorptivity at duodenum

jejunum and ileum were 0.85%-2.79%

0.70%-2.73% and 0.90%-1.77%

respectively.Quercitrin showed the highest absorption rate in duodenum and jejunum

polydatin showed the highest absorption rate in ileum.Absorption rate of oxyreverstroal was significantly slower than the other five ingredients

it just showed obvious absorption after 90 min in duodenum. Conclusion: Absorption of polydatin

astilbin

quercitrin

engeletin and resveratrol conforms the first order kinetics

everted gut sac method can evaluate intestinal absorption characteristics of multi-component in Smilacis Chinae Rhizoma extracts.

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