Objective: To investigate the protective effect of QingyiⅡ (QY) on the bile-duct ligation (BDL)-induced cholestatic liver injury. Method: Kunming mice were randomly divided into the sham operation group

model group

QY-0.5 group (0.5 g·kg-1) and QY-1.0 group (1.0 g·kg-1)

and orally administered for 7 days. On the 7th day

mice received BDL to prepare the cholestatic liver injury model. Livers and blood were collected 72 hours later after BDL. Serum enzyme biochemical indexes

histopathological changes and genes expression were determined. Result: Compared with sham operation group

there were significant increases in serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP)

increased total bile acid (TBA) and total bilirubin (TBIL) in model group. According to hepatic tissue pathological indexes

neutrophile infiltration and hepatocyte necrosis were apparent

the expression of cholesterol-7α-hydroxylase (Cyp7a1)

12α-hydroxylase (Cyp8b1)

farnesoid X receptor (FXR)

small heterodimer partner (SHP) and Ntcp mRNA expression were decreased (P<0.05). Compared with model group

QY decreased ALT

ALP activities and TBA

TBIL levels

alleviated the pathological injury

and up-regulated the expression of above genes (P<0.05). Conclusion: QY II could protect against cholestatic liver injury

and maintain the stability of bile acid by regulating gene expressions related to bile acid synthesis and transport in liver.