Objective: To investigate feasibility of application of zebrafish neuronal injury model on quality control for Yangxue Qingnao granules and to provide a new quality control method for this preparation. Method: Biological activity effect and stability of Yangxue Qingnao granules were quantitatively assessed in a zebrafish neuronal injury model which was established by mycophenolate mofetil (MMF). Result: Central nerve injury zebrafish model as a biological quality control standard for Yangxue Qingnao granules nerve cell protection exhibited excellent precision (intra-day RSD of 2.2%-3.7%

inter-day RSD of 1.2%)

repeatability (RSD of 8.4%) and stability (RSD of 4.9%).In the range of 0.1-1.0 g·L-1

there was a significant linear correlation;this method met requirements of biological activity determination of guiding principles. Conclusion: It can be turned out that methodology which established in dissertation is good at determining neuroprotective efficacy of Yangxue Qingnao granules

it is reliable

simple and repeatable

this method also provides a reference for development and improvement of bioassay of Chinese medicines.