Objective: To study the chemical components of Artemisiae Argyi Folium and their anti-hepatitis B virus(HBV) activities. Method: The chemical components were isolated and purified by silica gel column chromatography

and their structures were identified according to their physicochemical properties

nuclear magnetic resonance spectroscopy (NMR)

mass-spectrography (MS) and literature analysis. Furthermore

the toxicity of these chemical components to HepG 2.2.15 cells was measured by MTT method and their effects on HBeAg and HBsAg secretion in HepG 2.2.15 cell supernatant were measured by ELISA method. Result: Five compounds were isolated from Artemisiae Argyi Folium and their structures were identified as stigmasterol(1)

5

4'-dyhydroxyl-6

7

3'-trimethoxyflavone (3'-methoxythistle flavin) (2)

dammaradienylacetate(3)

5

7

4'-trihydroxy-3'

8-dimethoxyflavone(4)

and hexadecanoic dihydro ypropyleste(5). Conclusion: Compounds 2-4 were isolated from Artemisiae Argyi Folium for the first time and 3'-methoxythistle flavin showed inhibitory effect on HBsAg and HBeAg lines with IC50values of 8.09 mg·L-1 and less than 2.5 mg·L-1respectively.