Objective: To establish a HPLC method for simultaneous determinationof the content of epicatechin

vitexin

isovitexin

kaemferol-3-O-rutinoside

isorhamnetin-3-O-β-D-glucoside

and narcissi in extract of Microctis Folium. Method: The HPLC analysis was carried out on Agilent HC-C18column (4.6 mm×250 mm

5 μm) with amethanol-0.05% phosphoric acid solution as the mobile phase for gradient elution at a flow rate of 0.8 mL·min-1.The detection wavelength was 288 nm

and the column temperature was 35℃. Result: Good linear relationship were found in the the range of 131.200-1 312.000 ng for epicatechin

149.800-1 348.200 ng for vitexin

131.400-1 051.200 ng for isovitexin

99.175-595.050 ng for kaemferol-3-O-rutinoside

109.955-659.730 ng for isorhamnetin-3-O-β-D-glucoside

and 267.000-1 348.200 ng for narcissi. Their average recoveries were 98.13%

97.41%

96.93%

98.22%

97.37%

97.82%

respectively. Conclusion: The method is accurate

reliable

and can be used for the quality control and evaluationof total flavonoid extracts of Microctis Folium.