Objective: To establish an UPLC method for simultaneously determining content of 6 major compounds (chlorogenic acid

myricitrin

hyperoside

isoquercitrin

kaempferitrin

quercitrin) in Vicia amoena var. angusta. Method: An Eclipse Plus C18 RRHD column (2.1 mm×50 mm

1.8 μm) was adopted

with acetonitrile (B)-0.1% formic acid aqueous solution (A) as mobile phase for gradient elution (0-7 min

5%-10%B;7-11 min

10%-12%B;11-20 min

12%B;20-25 min

12%-19%B;25-30 min

19%-24%B). The flow rate was 0.2 mL·min-1. The detection wavelength was set at 340 nm and the column temperature was at 30℃. Result: Six components of V. amoena var. angusta showed good linear relationship in the linear range(r> 0.999 9) and the average recoveries were between 99.15% to 101.98% with the RSD of 0.7%-1.7%. Conclusion: The developed method is convenient

rapid and accurate with high repeatability and stability

suitable for simultaneously determining contents of 6 compounds in V. amoena var. angusta and can provide basis for the quality control of V. amoena var. angusta.