Objective: To establish HPLC fingerprint of Danggui Liuhuang Tang

analyze chromatographic data and analyze main components in Danggui Liuhuang Tang by high performance liquid chromatography and mass spectrometry (LC-MS) method. Method: HPLC was used on Agela Venusil XBP-C18(4.6 mm×250 mm

5.0 μm) column

with acetonitrile (A)-0.3% formic acid (B) as mobile phase for gradient elution (0-10 min

5%-15%A;10-15 min

15%-20%A;15-35 min

20%-25%A;35-50 min

25%-31%A;50-62 min

31%-55%A;62-70 min

55%-60%A;70-75 min

60%-70%A);with column temperature of 30℃

and volume flow rate of 0.8 mL·min-1;electrospray ionization was used for mass spectrometry

and full scan testing was done under cationic-anionic mode. Compounds were estimated through [M-H]-

[M+H]+ plasma information. Result: HPLC analysis and fingerprint were established for Danggui Liuhuang Tang;LC-MS spectra were established for Danggui Liuhuang Tang;19 characteristic chromatographic peaks of the Tang were identified. Conclusion: HPLC fingerprint analysis method of the Danggui Liuhuang Tang is stable and reliable

with good repeatability;the main components of Danggui Liuhuang Tang can be effectively identified through the establishment of LC-MS spectra.