Objective: To explore the molecular mechanisms of effective or toxic components of Kansui Radix and Glycyrrhizae Radix et Rhizoma by using network pharmacology method

and to investigate the correlations between multi-compounds

multi-targets and multi-pathways. Method: The main effective or toxic components of Kansui Radix and Glycyrrhizae Radix et Rhizoma

including kansuinine A

kansuinine B

kansuinine C

gycyrrhizic acid

liquiritin

isoliquiritin

formononetin were collected to build multi-components-proteins networks based on PharmMappear database. The obtained targets information was used to obtain corresponding pathways by using Boao MAS 3.0 system

and then Cytoscape software was used to construct components-targets-pathways network model. Result: The network analysis showed that

action of the effective or toxic components of Kansui Radix and Glycyrrhizae Radix et Rhizoma involved 61 pathways related to nerve-immune-endocrine-urinary. Contrary to their efficacy in adjusting water and electrolyte excretion

they were related with renin-angiotensin system

steroids biosynthesis and other pathways. Kansui Radix and Glycyrrhizae Radix et Rhizoma had synergistic effects in anti-tumor

anti-inflammatory and immune regulation

covering 33 pathways. Compatibility of Kansui Radix and Glycyrrhizae Radix et Rhizoma would enhance toxicity concerned to inhibition or induction of hepatic drug enzyme. Conclusion: Compatibility of Kansui Radix and Glycyrrhizae Radix et Rhizoma could result in decreased or synergistic effect and would change the toxicity. This reflected the compatibility mode of multi-compounds

multi-targets and multi-pathways for traditional Chinese medicines. It may provide reference for deeper study on molecular mechanism of compatibility of Kansui Radix and Glycyrrhizae Radix et Rhizoma.