Objective: To investigate whether entropy weight fuzzy comprehensive evaluation method is feasible for study on promoting effects of some kinds of penetration enhancers and to provide a reliable evaluation method for penetration enhancers. Method: Diclofenac sodium was used as model drug

azone

volatile oil from Citri Reticulatae Pericarpium

eucalyptus oil

menthol and borneol were tested as transdermal enhancers

taking isolated skin of SD rats as transdermal barrier

transdermal experiments were carried by a modified Franz diffusion cell.The content of diclofenac sodium was measured by HPLC with mobile phase of methanol-water-acetic acid (80: 20: 0.5) and detection wavelength at 276 nm.Permeability coefficient

lag time

steady flow and enhancement ratio were calculated.Effects of penetration enhancers were evaluated by entropy weight fuzzy comprehensive evaluation method. Result: Penetration enhancers had penetration enhancing effect on diclofenac sodium with different degrees.Azone

menthol and borneol had the best significant promoting effects whose maximum memberships were 0.761 1

0.582 1 and 0.495 4;volatile oil from Citri Reticulatae Pericarpium and eucalyptus oil had certain promoting effects whose maximum memberships were 0.454 2 and 0.480 9. Conclusion: Entropy weight fuzzy comprehensive evaluation method is feasible for evaluating promoting effects of penetration enhancers.