Objective: To study the chemical compositions of the fat-soluble alkaloids fraction of Aconitum tanguticum. Method: The fat-soluble alkaloids fraction was isolated and purified by using various modern chromatographic techniques

and the structures of the compounds were determined through physiochemical properties

spectral analysis (1D

2D-NMR and MS) and X-ray single crystal diffraction method. Result: Ten compounds were obtained from the fat-soluble alkaloids fraction of A. tanguticum

and their structures were identified as tanaconitine (1)

heteratisine (2)

11α

13α-dihydroxyhetisinone (3)

guan-fu base H (4)

β-sitosterol(5)

benzamide (6)

phthalic acid

butyl isobutyl ester (7)

dihydroatisine (8)

hetisine (9) and p-hydroxy phenylethanol (10). Conclusion: Compounds 1 -4

8 and 9 were diterpenoid alkaloids;compounds 3

6 -8 were isolated from the species for the first time. Moreover

the X-ray single crystal diffraction analysis of compounds 3 and 4 was done for the first time.