Objective: To provide scientific basis for the utilization and development of radix of Rubus reflexus var. lanceolobus by setting up the quality standards of radix of R. reflexus var. lanceolobus. Method: The properties and microscopic characteristics of radix of R. reflexus var. lanceolobus were observed. Extract

moisture and ash of the herbs from 10 different origins were determined according to the methods in Chinese Pharmacopoeia;qualitative research was done with TLC and their contents were determined by HPLC method. Result: The properties and microscopic characteristics of radix of R. reflexus var. lanceolobus were described;total ash was not more than 7%

acid-insoluble ash was not more than 4%

and moisture was not more than 11%for radix of R. reflexus var. lanceolobus. The spots of euscaphic acid were clear with no trailing in TLC;RSD was<2% in the stability and repeatability tests of euscaphic acid in content determination

and the content of euscaphic acid from 10 origins was 0.093 1%-0.244 3%

so it was temporarily determined that the euscaphic acid was not less than 0.08% in per gram of radix of R. reflexus var. lanceolobus. Conclusion: The established method can be used for the quality control of radix of R. reflexus var. lanceolobus.