Objective: To investigate the protective effects of Silibinin on acute liver injury induced by carbon tetrachlitied (CCl4) and D-galactosamine (D-Gal). Method: Totally 84 healthy Kunming mice were randomly divided into normal group

acute liver injury model group

silibinin low dose group

middle dose group and high dose group

and positive drug group. All the other groups except normal group received intraperitoneal injection of carbon tetrachlitied (0.2%) and galactosamine (800 mg·kg-1). 1 hour and 6 hours after modeling

silibinimin of different doses (120

240

480 mg·kg-1) was applied by the tail vein injection. Positive drug Tiopronin (40 mg·kg-1) was given for the acute liver injury induced by CCl4

and diammonium glycyrrhizinate (30 mg·kg-1) was given for the liver injury induced by D-Gal. 24 hours after modeling

the levels of aspartate transaminase (AST)

alanine transaminase (ALT) in serum

and superoxide dismutase (SOD)

malonal dehyde (MDA)

reduced glutathione (GSH) levels in liver homogenate were detected. Pathological changes of liver slices were investigated by htoxylin-eosin(HE) staining. Result: As compared with the normal group

the levels of AST

ALT and MDA were significantly increased in CCl4 and D-Gal models (P < 0.01);SOD and GSH levels were decreased significantly (P < 0.01). As compared with CCl4 and D-Gal model groups

the levels of AST

ALT and MDA were significantly reduced in the silibinin treatment groups

while the levels of SOD and GSH were increased (P < 0.01). Pathological results showed that the hepatic cells of the model group had obvious necrosis and lesions;the structure of hepatic lobule was still completed and no obvious lesion were found in hepatic cells after silibinin treatment. Conclusion: Silibinin could well protect the acute hepatic injury induced by carbon tetrachlitied and galactosamine.