Objective: To clarify pharmacodynamic material basis of salt-processed Alpiniae Oxyphyllae Fructus by investigating the contribution of chemical components from petroleum ether fraction of salt-processed products to the effect on detrusor in bladder of Guinea pig. Method: The fingerprint of petroleum ether fraction of salt-processed Alpiniae Oxyphyllae Fructus was established by HPLC

the mobile phase consisted of water and acetonitrile for gradient elution. Effect of petroleum ether fraction on detrusor in bladder of Guinea pig was investigated

and its material basis was analyzed

grey relational analysis method was used to establish the relationship between spectrum and efficacy. Result: The fingerprint of petroleum ether fraction of salt-processed products was established with similarity > 0.9. Detrusor inhibitory effect was the result of many kinds of components

the contribution of different peaks to relieve polyuria was ranked as 20 > 9 > 11 > 17 > 16 > 13 > 15 > 24 > 18 > 23 > 14 > 32 > 34 > 22 > 25 > 19 > 31 > 27 > 21 > 29 > 28 > 30. Conclusion: The HPLC fingerprints is simple and repeatable. The relationship between fingerprints of petroleum ether fraction and efficacy is paralleled to some extent

and this study will lay a foundation for the further research on inhibitory of salt-processed Alpiniae Oxyphyllae Fructus for polyuria.