Objective: To optimize preparation process of Shaoshang Yuhe hydrogel based on its pharmacodynamic index and determine the content of major pharmacological compositions in this preparation. Method: Hydrogels were prepared by different process

and the scald model in rats were used to evaluate efficacy by observing improvement of pathological structural and healing in wound area

measuring the leves of serum tumor necrosis factor-α

interleukin-1β and myeloperoxidase. HPLC and GC were utilized to measure the content of major pharmacological compositions in hydrogel and cream with the original process

respectively. Result: After treatment of 21 d

the wound areas of rats in Shaoshang Yuhe cream group

half extracted hydrogel group and Shirun Shaoshang cream group were (0.28±0.15)

(0.31±0.36)

(0.29±0.17) cm2

respectively. While effects of half extracted hydrogel group was better than Shaoshang Yuhe cream on reducing the infiltration degree of inflammatory cell and decreasing the content of three inflammatory factors

and it was closed to the Shirun Shaoshang cream group. The contents of three alkaloids as berberine hydrochloride

palmatine hydrochloride and coptisine hydrochloride in the hydrogel were 1.609

0.247

0.335 mg·g-1

respectively

there were higher than these in Shaoshang Yuhe cream. The contents of four volatile components as α-pinene

limonene

isoborneol and borneol were 0.052

0.149

1.438

1.643 mg·g-1

respectively

there were higher than these in the same batch of Shaoshang Yuhe cream. Conclusion: In addition to wound healing area

other pharmacodynamic indexes and the contents of effective components of the hydrogel by the optimized preparation process are better than the cream.