Objective: To study the chemical components from Prunellae Spica and their anti-tumor activities in vitro. Method: Extracts were separated and purified by column chromatography over silica gel

octadecylisilyl(ODS)

Sephadex LH-20

and the component structures were identified by physical and chemical properties and spectral methods including 1H-NMR and 13 C-NMR. MTT method was used to separately establish blank group

positive drug group (different concentrations of cisplatin)

and experimental drug groups at different concentrations;and measure the anti-tumor activities of these chemical components against MCF-7

MDA-MB-231 breast cancer cells and MCF-10A normal breast cell lines. Result: The 11 compounds were isolated and identified from the fruits of Prunellae Spica

and their structures were identified as:trans-salviaflaside (1)

trans-salviaflaside methyl ester (2)

rosmarinic acid (3)

3-(3

4-dihydroxyphenyl)acrylic acid 1-(3

4-dihydroxy-phenyl)-2-methoxycarbonylethylester (4)

caffeic acid-3-O-glucoside (5)

danshengsu (6)

3

4

α-trihydroxy-mephenylpropionate (7)

3

4-dihydroxybenzaldhyde (8)

(3R

5S

6S

7E

9R)-megastigman-7-ene-3

5

6

9-tetrol-9-O-β-D-glucopy-ranoside (9)

(-)-syringaresinol-4-O-β-D-glucopyranoside (10)

and 16-oxygen-17-demethyl-ation-3β

24-hydroxy oleanolic acid-12-en-3-O-β-D-glucuronic acid (11). Compound 4 showed an inhibitory rate over 88% against all the cells

compounds 2 and 8 showed an inhibitory rate over 60% against the cell lines MCF-7 and MCF-10A

compound 3 showed an inhibitory rate over 60% only against the MCF-7. Conclusion: Compounds 9 and 10 were obtained from the Prunellae Spica for the first time. The results of anti-tumor assay indicated that compounds 2-4 and 8 had significant inhibitory effect against breast cancer cell MCF-7

compound 4 had strong inhibitory effect against breast cancer cell MDA-MB-231;but the compounds 2

4 and 8 also showed inhibitory effect against normal breast cell lines

and compound 3 can selectively inhibit the cancer cells.