Objective: To establish a HPLC method for the determination of agaropiric acid in artificial agarwood and study the correlation between agaropiric acid and extract contents. Method: The content of ethanol-soluble extract was determined according to those correlative standards of Aquilariae Lignum Resinatum in Chinese Pharmacopoeia (2015 Version);HPLC was done on CNW Athena C18-WP column (4.6 mm×250 mm

5 μm)

with acetonitrile-0.1% phosphoric acid solution (40: 60) as the mobile phase at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 217 nm and the column temperature was 35℃. Result: The linear range of agaropiric acid was 0.24-24 mg·L-1 (r=0.999 7). RSD was 0.4% for precision degree and 0.9% for stability within 24 h

the average mass fraction of agaropiric acid was 27.86 μg·g-1

and its average recovery rate was 96.4% with RSD of 2.8%. Conclusion: This HPLC method is accurate for determination of agaropiric acid content. The average content of agaropiric acid was highest in comprehensive way

and the content of agaropiric acid is increased with the increase of ethanol-soluble extract content. This correlation can provide the relativity of contents between the ethanol-soluble extract and agaropiric acid

and provide a reference for quality evaluation of artificial agarwood.