Objective: To establish a method for determining the contents of quercetin

caffeic acid

luteolin

and acacetin in Uygur Medicine Hyssopus cuspidatus. Method: Agilent C18 chromatographic column (4.6 mm×250 mm

5 μm) was used with methanol-0.2% acetic acid solution as the mobile phase. The detection wavelength was set at 340 nm and the flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the injection volume was 10 μL. Result: Quercetin

caffeic acid

luteolin

and acacetin showed good linear relationship in 0.120 2-1.081 8 mg(r=0.998 6)

0.031-0.279 mg(r=0.999 9)

0.052-0.468 mg(r=0.998 9)

and 0.098 1-0.882 9 mg(r=0.998 8) respectively

and their recovery rate was 98.62%(RSD 1.4%)

97.38%(RSD 1.7%)

99.41%(RSD 1.5%)

and 99.81%(RSD 1.0%) respectively. Conclusion: This RP-HPLC method is feasible and simple for simultaneous determination of 4 components in H. cuspidatus

which can provide theoretical indicators for the content determination of H. cuspidatus.