大鼠尿液中秦皮甲素和秦皮乙素代谢物的HPLC-MS分析

杨建华; 王晓梅; 迪丽拜尔·; 马木提; 杨秀娟; 胡君萍

doi:10.13422/j.cnki.syfjx.2016110093

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大鼠尿液中秦皮甲素和秦皮乙素代谢物的HPLC-MS分析

药物代谢 | 更新时间：2024-01-04

- 大鼠尿液中秦皮甲素和秦皮乙素代谢物的HPLC-MS分析
- Study on Metabolites of Aesculin and Aesculetin in Rat Urine by HPLC-MS
- 中国实验方剂学杂志 2016年22卷第11期页码：93-96
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30960526,81560688)
- DOI：10.13422/j.cnki.syfjx.2016110093
  中图分类号： R285.5
- 纸质出版日期：2016
- 稿件说明：
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杨建华, 王晓梅, 迪丽拜尔·, 等. 大鼠尿液中秦皮甲素和秦皮乙素代谢物的HPLC-MS分析[J]. 中国实验方剂学杂志, 2016,22(11):93-96.

YANG Jian-hua, WANG Xiao-mei, Dilibar·, et al. Study on Metabolites of Aesculin and Aesculetin in Rat Urine by HPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 93-96.
杨建华, 王晓梅, 迪丽拜尔·, 等. 大鼠尿液中秦皮甲素和秦皮乙素代谢物的HPLC-MS分析[J]. 中国实验方剂学杂志, 2016,22(11):93-96. DOI： 10.13422/j.cnki.syfjx.2016110093.

YANG Jian-hua, WANG Xiao-mei, Dilibar·, et al. Study on Metabolites of Aesculin and Aesculetin in Rat Urine by HPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(11): 93-96. DOI： 10.13422/j.cnki.syfjx.2016110093.

摘要

目的: 探索秦皮乙素和秦皮甲素在大鼠尿液中的代谢物

以期阐明这2种药物在体内作用的化学基础。方法: 单次灌胃给药秦皮乙素和秦皮甲素后收集48 h内大鼠尿液

经固相萃取法处理

采用HPLC-MSn检测代谢产物

色谱条件为流动相甲醇-0.2%甲酸(12:88)

流速1.0 mL·min-1

进样量5 μL

柱温35℃

检测波长339 nm;质谱条件为电喷雾离子化方式

正离子扫描模式

喷雾电压3.0 kV

碰撞气流量0.16 mL·min-1

洗脱溶剂温度350℃

离子源温度450℃。结果: 从大鼠尿液中发现并初步鉴定了5个代谢产物的化学结构

主要为秦皮乙素的水解产物、还原产物、甲基化产物及硫酸化产物。结论: 推测秦皮甲素在体内先水解为秦皮乙素

再进行水解、还原、甲基化及硫酸化反应。

Abstract

Objective: To investigate the metabolites of aesculin and aesculetin in rat urine. Method: After single oral administration of aesculin and aesculetin to rats

urine was collected within 48 h and pretreated by solid-phase extraction method. Metabolites were determined by a HPLC-MSn method. Result: Five major metabolites were found and their chemical structures were elucidated as hydrolysate

reduzate

methylated metabolite and sulfated product

respectively. Conclusion: According to the rule of metabolism

aesculin is hydrolyzed as aesculetin which were further metabolized in vivo. The method can be used to identify the structures of active components responsible for pharmacological effects.

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