Objective: To observe the effects of iridoid glycoside extract from Corni Fructus (ECF) on mitochondrial oxygen consumption and related genes expression in 3T3-L1 mature adipocytes

and analyze the possible molecular mechanism. Method: With 3T3-L1 adipocytes as the study objects

Seahorse XF Cell Mito Stress Test was used to detect the effect of ECF (at the concentrations of 0.04

0.2

1 g·L-1) for 2 d on the oxygen consumption rate in adipocytes

and blank group was additionally set up. Peroxisome proliferators-activated receptor-γ coactivator-1α (PGC-1α)

silent information regulator 2 related enzyme 1 (SIRT1)

adenosine monophosphate activated protein kinase (AMPK)

mitochondrial uncoupling protein 1 antibodies (UCP-1)

PR domain proteins 16 (PRDM16) and peroxisome proliferators-activated receptor γ (PPARγ) mRNA expressions were detected by reverse transcription-polymerase chain reaction (RT-PCR). Result: As compared with the blank group

2 days' intervention of 0.2

1 g·L-1 ECF in mature 3T3-L1 adipocytes enhanced ATP production

basal respiration and maximal respiration;1 g·L-1 ECF intervention enhanced the spare respiration capacity;0.04 g·L-1 ECF significantly increased the oxygen consumption rate of proton leak. ECF intervention for 2 days at various concentrations significantly up-regulated PGC-1α

AMPK and SIRT1 mRNA levels

and significantly down-regulated PPARγ mRNA expression level

with statistically significant differences (P < 0.05

P < 0.01)

but they showed no significant effects on UCP-1 and PRDM16 mRNA expression levels. Conclusion: In mature adipocytes

ECO could enhance the aerobic respiration and increase ATP production

and the adipocytes have no beiging characteristics

so these effects might be related to regulations of AMPK

SIRT1

PGC-1α and PPARγ levels.