Objective: To truly realize quality control of concentration process of Gardeniae Fructus extracting solution

near-infrared spectroscopy (NIR) was used to establish a rapid quantitative analysis model for contents of chlorogenic acid and geniposide

total solid content and density during concentrating process of Gardeniae Fructus extracting solution

which could real-timely reflect its state. Method: Concentration solution samples of Gardeniae Fructus extracting solution in the workshop were collected and on-line NIR were acquired simultaneously. After rejection of abnormal samples

pretreatment of spectra and choice of the corresponding wave band

the multivariate calibration models were respectively established using partial least square method (PLS) between spectra and chlorogenic acid content

geniposide content

total solid content and density

then these models were measured by reference method

which could be used to predicte unknown samples. Result: Correlation coefficients of contents of chlorogenic acid and geniposide

total solid content and density in calibration set were 0.990 5

0.994 3

0.986 2 and 0.987 9;correlation coefficients in validation set were 0.987 2

0.992 5

0.981 6 and 0.982 3;root mean square errors of cross-valibration (RMSECV) were 0.112

0.368

0.148 and 0.013

root mean square error of prediction (RMSEP) were 0.181

0.302

0.128 and 0.016

respectively. Relative standard errors of predictions (RSEP) were lower than 6.0%

which can satisfy the quality control standard in production process of traditional Chinese medicine. Conclusion: The method is simple

accurate

reliable and applicable for fast analysis and monitoring of contents of chlorogenic acid and geniposide

total solid content and density in concentration process of Gardeniae Fructus extracting solution from Reduning injection.