Objective: To establish a HPLC-DAD method for simultaneous determination of 14 active compounds (baicalin

oroxyloside

wogonoside

jatrorrhizine

copetisine

palmatine

berberine

baicalein

rhein

aloe-emodin

wogonin

emodin

chrysophanol and physcion) in Yiqing capsules. Method: Agilent 1200 HPLC-DAD chromatographic instrument and Agilent ZORBAX SB-C18 chromatographic column (4.6 mm×250 mm

3.5 μm) were used

with water-acetonitrile-0.2% formic acid in methanol-25 mmol·L-1 KH2PO4 solution as the mobile phase for gradient elution. The flow rate was 0.8 mL·min-1

with the column temperature of 25℃

and DAD detection wavelength was 254

275

345 nm respectively. Result: Fourteen compounds showed good linear relationship; the recovery rate was ranged from 97.11%-102.44% with RSD less than 3.0% for all the analytes (n=6). RSD of precision degree was less than 3.0%; and RSD of repeatability testing results was less than 3.0%. Conclusion: This HPLC-DAD method is simple

accurate and reproducible. It could provide scientific basis for the quality control of Yiqing capsules.