Objective: To discuss the effect of Shenling Baizhu particles on intestinal epithelial tight junctions in rats with functional diarrhea. Method: The functional diarrhea spleen deficiency syndrome model in rats was established by using high lactose+small platform standing method. the rats were divided into normal group

model group

pinaverium bromide group

Shenling Baizhu particle high

medium and low dose groups. Shenling Baizhu particle groups were administrated with Shenling Baizhu particle at doses of 3.212

1.606

0.803 g·kg-1

respectively

and pinaverium bromide group was given pinaverium bromide of 0.0125 g·kg-1; meanwhile

normal group and model group were given physiological saline intragastrically for 14 successive days. The diamine oxidase(DAO) and tumor necrosis factor-α(TNF-α) were detected by enzyme-linked immunosorbent assay(ELISA) method; the zonula occludens-1 (ZO-1)

Claudin-1

Occludin mRNA expressions were detected by real-time fluorescent quantitative polymerase chain raction(qPCR) and ZO-1

Claudin-1 protein expressions were detected by immunohistochemistry method. Result: Compared with the normal group

the model group showed significant increases in serum DAO and TNF-α content in colonic mucosa (P<0.01)

and decreases in ZO-1

Occludin

Claudin-1 mRNA expressions (P<0.01) and ZO-1

Occludin protein expression (P<0.01); compared with the normal group

Shenling Baizhu particle groups showed decreases in serum DAO and TNF-α content in colonic mucosa (P<0.01)

and up-regulations in ZO-1

Occludin

Claudin-1 mRNA expressions (P<0.01) and ZO-1

Occludin protein expression P<0.05

P<0.01) in functional diarrhea model rats. Conclusion: Shenling Baizhu particles can regulate tight junction protein expression in colonic mucosa

and intestinal mucosal mechanical barrier

which may be one of mechanisms of treating functional diarrhea.