Objective: To observe the effect of Hui medicine Zhali Nusi Fang (ZLNF) in intervening the impact of bone marrow mesenchymal stem cells (BMSCs) transplantation on neuronal axons regeneration in rats after cerebral ischemia reperfusion injury (CIRI). Method: SD rats were divided randomly into Sham-operated group

model group

ZLNF group

BMSCs transplantation group and ZLNF-BMSC combination group; middle cerebral artery occlusion (MCAO) model was duplicated with nylon thread

BMSCs were cultured and amplified by the whole bone marrow adherence method; drugs were given to the rats by intragastric administration (14.6 g·kg-1·d-1)

BMSCs were transplanted into rat brains through carotid artery; rat brains were taken out at 1

3

7

14 days after transplantation; growth associated protein-43 (GAP-43)

neurofilament protein-200 (NF-200)

myelin associated glycoprotein (MAG) and Nogo-A protein expression were detected by Western blot. Result: The GAP-43

MAG

Nogo-A in the model groups were obviously increased than the sham-operated groups (P<0.01)

and with significant decrease in NF-200 (P<0.01); compared with the model groups

the GAP-43 at 3

14 days in the ZLNF group

at 3

7

14 days in the transplantation group and the combination group were increased (P<0.01

P<0.05)

the NF-200 in the ZLNF group

the transplantation group and the combination group were increased (P<0.01)

but with decreases in MAG and Nogo-A (P<0.05); compared with the transplantation groups

the GAP-43 at 14 day

the NF-200 at 7 day and MAG at 1

3

14 days in the ZLNF group were decreased (P<0.01

P<0.05)

and the Nogo-A at 3 day in the ZLNF group was decreased (P<0.01) but increased at 7 day (P<0.05)

the GAP-43 at 1

14 days and the NF-200 in the combination group were increased (P<0.01)

the NF-200 at 7 day and MAG

Nogo-A in the combination group were decreased (P<0.01); compared with the ZLNF group

the GAP-43 at 3

7

14 days and the NF-200 at 14 day in the combination group were increased(P<0.01)

the MAG

Nogo-A in all of the groups were decreased (P<0.01); in the intra-group comparison

the GAP-43 and MAG at 7 day in the model group were increased compared with that at 3

14 days (P<0.01

P<0.05)

the NF-200 at 3 day in the model group was increased compared with that at 1 day

the Nogo-A at 3 day reached peak (P<0.01)

the GAP-43 at 14 day group in ZLNF

transplantation and combination groups were higher than that at 7 day (P<0.01)

the NF-200 at 7 day in ZLNF and transplantation groups were higher than that at 3

14 days (P<0.01

P<0.05)

the NF-200 at 3 day in the combination group was relatively low (P<0.01) and later gradually higher (P<0.05)

the MAG

Nogo-A in ZLNF

transplantation and combination groups were increased first and then decreased. Conclusion: Hui medicine ZLNF can obviously promote the neuronal axons regeneration after the transplantation of BMSCs for cerebral ischemia reperfusion injury

with a significant effect in their combination

and its mechanism may be related to dynamic expressions of GAP-43

NF-200

MAG and Nogo-A after the intervention.