Objective: To isolate balsamic acid esters from benzoin

identify the structures and preliminarily study their efficacy. Method: Benzoin was extracted by 95% ethanol

further separated and purified by column chromatography; the isolated compounds were identified by1H-NMR and 13C-NMR data. The efficacy of these isolated compounds was studied. Result: Isolated compound from benzoin was identified as coniferyl benzoate. Pharmacological experiments showed that the coniferyl benzoate and benzoin group exhibited similar effects

both of which significantly increased evans blue content in the brains of normal mice and exhibited protective effect on endotoxin fatal attack in D-galactosamine-sensitized mice. Conclusion: Coniferyl benzoate is an important component in total balsam acid esters of benzoin and has significant correlation with efficacy of crude benzoin.