Objective: To establish GC-MS fingerprint of Clerodendranthus spicatus volatile components. Method: Volatile components in twelve batches of C. spicatus samples were analyzed by GC-MS method combined with temperature-programmed retention index technique. Research was performed on DB-5MS chromatographic column; carrier gas was helium (He)

its flow rate was 1.0 mL·min-1

and the injection volume was 1 μL

the refractive ratio of distillation was 100:1.MS conditions were as follows:electron impact (EI) ion source

transmission line temperature of 280℃

ion source temperature of 230℃

quadrupole temperature of 150℃

and mass scan range of m/z 30-600. Result: Thirteen common peaks were identified in the GC-MS fingerprint of volatile components from 12 batches of C. spicatus samples. Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica(Version 2004 A) was used for fingerprint analysis. Similarity of 12 batches of C. spicatus samples was higher than 0.999. Conclusion: GC-MS combined with temperature-programmed retention index can more comprehensively reflect the volatile components of C.spicatus. The established GC-MS fingerprint is highly characteristic and specific

which can be used as an effective method for quality control of volatile components of C.spicatus.