Objective: To establish a HPLC method for simultaneously determining the contents of chlorogenic acid

gardenoside

paeoniflorin and liquiritin in Lingyang Qingfei powder. Method: A double wavelength HPLC method was performed on Kromasil C18 column (4.6 mm×250 mm

5 μm) with acetonitrile and 0.1% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was set at 1.0 mL·min-1 and the column temperature was 28℃. The detector wavelength was 327 nm for chlorogenic acid and 237 nm for gardenoside

paeoniflorin and liquiritin. Result: The chlorogenic acid

gardenoside

paeoniflorin and liquiritin showed good linear relationship with peak area at the sample size of 1.579-78.96 μg(r=0.9998)

1.261-63.04 μg(r=1)

0.364-18.2 μg(r=0.9999)

and 0.329 6-16.48 μg(r=0.9999) respectively. The average recovery rate was 97.6%

97.6%

97.1% and 99.8% respectively for the above components

with RSD of 1.0%

0.7%

1.2% and 1.3%

respectively. Conclusion: The method is simple

rapid and accurate

and can be used for the quality control of Lingyang Qingfei powder.