Objective: To discuss the molecular mechanism of Biejiajian Wan in inhibiting hepatocellular carcinoma by studying its effect on the signaling molecules of Wnt/β-catenin signaling pathway and the expression of target genes on HepG2 cell xenograft in nude mice. Method: The hepatocellular carcinoma mode was established by tumor formation experiment in nude mice

and the mice were randomly divided into the high dose group (2.2 g·kg-1)

the middle dose group (1.1 g·kg-1)

the low dose group (0.55 g·kg-1)

the positive control group

and the normal control group. According to the visual observation

Biejiajian Wan had an inhibitory effect on the growth of implanted tumors of HepG2 cell xenograft in nude mice. Expressions of β-catenin and T-box 3 (TBX3) of HepG2 cell xenograft were measured by the immunohistochemical method. Expressions of p19ARF

murine double minute 2 (MDM2) and p53 in HepG2 cell xenograft were tested by Western blot. Result: Biejiajian Wan can significantly inhibit the growth of the implanted tumor of HepG2 cell xenograft in nude mice

and the expressions of β-catenin and TBX3 (P < 0.05)

as well as the expression of the p19ARF

p53 and MDM2 (P < 0.05). Conclusion: Biejiajian Wan can regulate the expression of β-catenin and TBX3 by affecting the Wnt/β-catenin signaling pathway

and then activate the cancer suppressor gene p53 by regulating the TBX3/p19ARF/MDM2 signaling pathway. Finally

Biejiajian Wan can repress the growth of liver cancer cells and induce cell apoptosis

so as to resist hepatocellular carcinoma.