Objective: To study and explore the effect and mechanism of Kuan Quzhen gelatum on rat model with eczema. Method: The eczema model of rat was established with 2

4-dinitrochlorobenzene. Totally 40 rats were divided into four groups

namely normal group

eczema group

positive control group and Kuan Quzhen gelatum group. The effect was observed by skin lesion score. Pathological changes were observed by paraffin sections. Interferon-γ(IFN-γ)

interleukin-4(IL-4)

interleukin-17 (IL-17)

interleukin-31 (IL-31)

leukotriene B4 (LTB4) and leukotriene C4 (LTC4) were measured by enzyme-linked immunosorbent assay. The expressions of cysteinyl leukotriene receptor 1 (CysLTR1) and cysteinyl leukotriene receptor 2 (CysLTR2) were detected by immunohistochemistry method and Western blot. Result: Compared with normal group

eczema model group showed hyperkeratosis

parakeratosis

hypergranulosis and acanthosis

with increases in IL-4

IL-17

IL-31

LTB4 and LTC4 and up-regulation in the expressions of CysLTR1 and CysLTR2.Compared with eczema model group

Kuan Quzhen Gelatum group showed reductions in skin lesion score after 6 days

pathological changes

and the level of IL-4

IL-17

IL-31 and LTC4 (P < 0.05)

and down-regulations in the expressions of CysLTR1 and CysLTR2

with no statistically significant difference in the level of LTB4. Conclusion: Kuan Quzhen Gelatum has therapeutic effects on rat model with eczema

which may be related with declines in IL-4

IL-17

IL-31 and LTC4 levels in serum and down-regulation in CysLTR1 and CysLTR2 expressions.